RP-HPLC Method for Simultaneous Determination of Paracetamol, Diclofenac Sodium and Famotidine in Tablet Dosage Form
Maslarska VN*, Tsvetkova BG, Peikova LP
A simple, precise and accurate liquid chromatographic method was described for simultaneous determination of paracetamol, diclofenac sodium and famotidine in tablets. The chromatography was carried out on a C18 (250 mm x 4.6 mm, 10 μm) column with acetonitrile:water: 0.5% trimethylamine : o-phosphoric acid (60:20:5:15v/v) as mobile phase, at a flow rate of 1.0 ml/min, with detection at 240 nm. Separation was completed in less than 10 min. The calibration curves were linear in the concentration range of 125.0-1000.0 μg/ml for paracetamol, 25.00-200.0 μg/ml for diclofenac sodium and 5.00-40.00 μg/ml for famotidine. The intra- and inter-day relative standard deviations for both the components were <2.0 %. The results of the studies showed that the proposed RP-HPLC method is rapid, precise and accurate, which can be applied for the routine assessment of described drugs in pharmaceutical dosage forms.
Paracetamol, Diclofenac Sodium, Famotidine, Simultaneous Determination, RP-HPLC, Validation
Cite This Article
Maslarska, V. N., Tsvetkova, B. G., & Peikova, L. P. (2015). RP-HPLC Method for Simultaneous Determination of Paracetamol, Diclofenac Sodium and Famotidine in Tablet Dosage Form, International Journal for Pharmaceutical Research Scholars, 4(2), 55-61.