RP-HPLC Methods Development for the Simultaneous Estimation of Quinine and Ciprofloxacin

RP-HPLC Methods Development for the Simultaneous Estimation of Quinine and Ciprofloxacin


Sawant RL, Jadhav KA*, Tanpure KD, Bharat AV


Abstract

A simple, rapid, sensitive and specific liquid chromatographic method with UV detection for the simultaneous estimation of quinine and ciprofloxacin was developed. Separation was achieved with HibarR 250-4.6 HPLC column PurosphensR STAR RP-18 and mobile phase containing acetonitrile: methanol: triethylamine (0.1%) in double grade distilled water in the ratio 1:6:3 (pH was adjusted with ortho-phosphoric acid as 3.8) at flow rate 1 ml/min. Quantitation was achieved with UV detector at 236 nm. The selected chromatographic conditions effectively separated quinine and ciprofloxacin with retention time of 4.383 and 3.042 min. respectively. The developed method is precise, accurate, reproducible and specific.


Keywords

Ciprofloxacin Hydrochloride, Reverse Phase High Performance Liquid Chromatography, Quinine Sulphate


Cite This Article

Sawant, R. L., Jadhav, K. A., Tanpure, K. D., & Bharat, A. V. (2015). RP-HPLC Methods Development for the Simultaneous Estimation of Quinine and Ciprofloxacin, International Journal for Pharmaceutical Research Scholars, 4(2), 130-135. 

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