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Development and Validation of Spectrofluorimetric and RP-HPLC with Fluorimetric Detection Methods for the Determination of Vilazodone in Bulk and Pharmaceutical Preparation


Received On: 09/04/2014
Accepted On: 17/04/2014


Author(s)

El-Bagary, R. I., Hashem, H. A. E. M., Fouad, M. A., Mahmoud, S. T.


Author's Affiliation


Abstract

Two simple, rapid, and sensitive methods, namely, spectrofluorimetric and high performance liquid chromatographic (HPLC) methods were developed for the determination of Vilazodone HCl. The first method is based on measuring the native fluorescence of the cited drug at its optimum emission wavelength. The fluorescence intensity was measured at λem 486 nm upon excitation at λex 353 nm. The calibration graph was linear over the concentration range 2–8 μgmL−1. The second method is based on isocratic elution of Vilazodone HCl on reversed phase Supelco C18 column, (25 cm, 4.6 mm, 5 µm), using a mobile phase consisting of 0.02 M Sodium hydrogen phosphate adjusted to pH 5 with o-phosphoric acid: methanol (30:70,v/v), at a flow rate of 1 mLmin-1. Quantitation was achieved using spectrofluorimetric detection where the fluorescence intensity of Vilazodone HCl was detected at λem 486 nm upon excitation at λex 353 nm at ambient temperature. The methods were validated according to the International Conference on Harmonization (ICH) guidelines. The proposed methods were applied to the determination of the cited drug both in bulk and pharmaceutical preparation.


Keywords

Spectrofluorimetry, Vilazodone HCl, Native fluorescence, Pharmaceutical preparation, RP-HPLC

Cite This Article

Ramzia Ismail El-Bagary, Hanaa Abd El Monaim Hashem, Marwa Ahmed Fouad, Sally Tarek Mahmoud. (2014). Development and Validation of Spectrofluorimetric and RP-HPLC with Fluorimetric Detection Methods for the Determination of Vilazodone in Bulk and Pharmaceutical Preparation. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 113-119.


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