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Stability Indicating HPLC Method for Simultaneous Determination of Ketoprofen and Methylparaben in Gels


Received On: 20/05/2014
Accepted On: 24/05/2014


Author(s)

Maslarska, V.


Author's Affiliation


Abstract

A simple and fast reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the simultaneous determination of ketoprofen – an active component and methylparaben – a preservative, presented in the 2.5 % Ketoprofen gel. The chromatographic separation was performed on a LiChrosorb C18 column; the mobile phase for separation of these compounds consisted of a mixture of 140 ml acetonitrile: methanol (60:40 v/v) and 160 ml 0.5% ammonium acetate. The pH was adjusted to 5.9 with 10 % nitric acid. The analysis time was less than 5 min, at a flow rate 2.0 ml/min and detection at 254 nm. The method was found to be applicable for routine analysis (stability tests, homogeneity) in the pharmaceutical product – Ketoprofen gel 2.5%.


Keywords

Ketoprofen, Methylparaben, Preservatives, HPLC, Validation

Cite This Article

Maslarska, V. (2014). Stability Indicating HPLC Method for Simultaneous Determination of Ketoprofen and Methylparaben in Gels. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 571-576.


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