Received On: 16/04/2018
Accepted On: 02/05/2018
Vaishnavi, K. G., Varsha, S. T., Nirmal, M. T., Somnath, A. P., Mallinath, S. K.
(Vaishnavi, K. G., Mallinath, S. K.)1,5, Department of Quality Assurance, D.S.T.S. Mandal’s College of Pharmacy, Solapur, Maharashtra, India. (Varsha, S. T.)2, Department of Pharmaceutical Chemistry, D.S.T.S. Mandal’s College of Pharmacy, Solapur, Maharashtra, India. (Nirmal, M. T.)3, Research Scholar, Parul University, Parul Institute of Pharmacy, P.O Limbda, Waghodia, Vadodara, Gujarat, India. (Somnath, A. P.)4, Department of Pharmaceutical Analysis, D.S.T.S. Mandal’s College of Pharmacy, Solapur, Maharashtra, India.
Simple, rapid, accurate and precise UV spectrophotometric and RP-HPLC methods had been developed for simultaneous estimation of Sitagliptin Phosphate (STG) and Pioglitazone Hydrochloride (PIO). The method applied was Simultaneous Equation Method (Vierodt’s Method), based on measurement of absorbance of Sitagliptin Phosphate and Pioglitazone Hydrochloride at λmax 267nm and 270nm respectively. Linearity was found in the concentration range of 50-250µg/ml for Sitagliptin and 5-25µg/ml for Pioglitazone with regression coefficient r2=09997 and r2=0.9995 respectively. RP-HPLC method was carried on Phenomenex C-18 column (150 mm × 4.6 mm, 5 m) by using a mobile phase acetonitrile: methanol: water (30:30:40) as a mobile phase at 1.0 ml/min flow rate at 270 nm. The linearity was found to be in the range of 10-50 µg/ml and 3-15µg/ml with regression coefficient of r2= 0.9998, and r2=0.9996 for Sitagliptin Phosphate and Pioglitazone HCl respectively. The peak obtained were sharp having clear baseline separation with a retention time 5.6 and 2.8 min for Sitagliptin Phosphate and Pioglitazone HCl. This method is accurate and precise and can be employed for routine analysis of Sitagliptin Phosphate and Pioglitazone hydrochloride in different pharmaceutical dosage forms.
Sitagliptin Phosphate, Pioglitazone HCl, UV- spectrophotometry, RP-HPLC
Cite This Article
Vaishnavi, K. G., Varsha, S. T., Nirmal, M. T., Somnath, A. P., Mallinath, S. K. (2018). Development and Validation of Analytical Methods for Simultaneous Estimation of Sitagliptin Phosphate and Pioglitazone Hydrochloride. International Journal for Pharmaceutical Research Scholars (IJPRS), 7(2), 12-22.