Home Abstract

A Review on Method Development and Validation by Using HPLC

Received On: 21/07/2016
Accepted On: 31/07/2016


Mishra, K., Sing, B.K.

Author's Affiliation


Chromatography, although primarily a separation technique, is mostly employed in chemical analysis, in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. This article involves the strategies and the issues for designing HPLC method development and validation. HPLC methods should be able to separate, detect and quantify the various drug-related impurities that may be introduced during synthesis. The method development often follows the well established steps, like selection of buffer, selection mobile phase, selection of column. Method validation includes the parameters like accuracy, precision, linearity, LOQ and LOD, specificity etc. A number of chromatographic parameters were evaluated in order to optimize the method.


HPLC, Method development, Validation, Impurity, Chromatography

Cite This Article

Mishra, K., Sing, B.K. (2016). A Review on Method Development and Validation by Using HPLC, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(3), 71-81.

Recent Articles

Get FREE Updates for Upcoming
Pharma News, Jobs & Conferences

You have successfully subscribed to the newsletter

There was an error while trying to send your request. Please try again.

IJPRS will use the information you provide on this form to be in touch with you and to provide updates and marketing.