Abstract

Antibiotics are commonly used to treat most microbial infections. But their irrational use, resistance of organism towards them and their side-effects have put their significance in jeopardy. Medicinal plants are known to cure microbial diseases since ancient times and exhibit minimum side effects. In the present study an attempt has been made to study antibacterial activity of Crataeva tapia L. (leaf and bark extracts) against Escherichia coli 2184, Proteus mirabilis 2241, Bacillus subtilis 2063 and Staphylococcus aureus 2079 procured from National Collection of Industrial Microorganisms (NCIM, Pune). Petroleum ether, ethanol and aqueous extracts of leaf and bark were used to evaluate the antibacterial activity of Crataeva tapia L. Antibacterial activity was established by determining Minimum Inhibitory Concentration (MIC) of leaf and bark extracts followed by agar well diffusion method. For agar well diffusion method, Ciprofloxacin (Ciplox), a broad spectrum antibiotic and sterile Nutrient Broth served as positive and negative control respectively. The results obtained from the present study revealed potential use of the plant for developing antibacterial compounds against tested bacteria.

Keywords

Crataeva tapia L., Staphylococcus aureus 2079, Bacillus subtilis 2063, Escherichia coli 2184, Proteus mirabilis 2241, Antibacterial, MIC, Agar Diffusion

Cite This Article

Sharma, P., Patil, D., Dhaliwal, M. K., & Patil, A. (2014). Antibacterial Activity of Leaf and Bark Extracts of Crataeva Tapia L. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(4), 28-40.