Abstract

A study was carried out on the population study of Staphylococcus aureus with 3 different carbon sources. The treatments in the study involved ferulic acid (FA), ferulic acid + glucose (FAG) and ferulic acid + biosludge (FAB). The study was monitored with an incubation period of 10 days at room temperature. The purpose of study was to detect the effect of glucose and biosludge amendments into the medium during the biotransformation of ferulic acid into vanillin using S. aureus. Results revealed that S. aureus consumed ferulic acid rapidly with more than 4-fold increased accumulation of vanillin (45.7 mg/l) on day 2 in FAG treatment, whereas 9.8 mg/l of vanillin accumulation was found on day 7 in FA treatment.  FAB treatment gave an intermediate effect on day 4 with vanillin accumulation of 22.8 mg/l. Hence, as per the production of vanillin, the treatments can be ranked as FAG > FAB > FA. A large staphylococcal load was detected in FAG treatment on day 2 of incubation due to supplementation of glucose. In FA treatment, their population was lowest among all conditions. After day 2, S. aureus population in FAG treatment was found to decrease rapidly whereas a steady decrease was found in FAB.

Keywords

S. Aureus, Ferulic Acid, Glucose, Biosludge, Vanillin

Cite This Article

Sarangi, P. K., Sahoo, H. P., Nanda, S., Pattnaik, B., & Das, M. (2014). Biocatalytic Activity as a Function of Staphylococcus aureus Population with Different Carbon Sources during Ferulic Acid Degradation. International Journal for Pharmaceutical Research Scholars, 3(1), 388-391.