Abstract

A high performance liquid chromatographic method was developed, validated and applied for determination of Nicergoline in pharmaceutical formulations. A LiChrosorb^® RP-18 (10 µm, 250x 4 mm) column was used with a mobile phase consisting of acetonitrile: methanol: phosphate buffer (40: 35: 25 % v/v, pH 7.0), a quantitative evaluation was performed at 288 nm with flow rate of 1.5 mL/min, and column cooler temperature was maintained at 25 ºC. The retention time was about 8 min. Suitability of this method for the quantitative determination of the drug was proved by validation in accordance with the requirements laid down by the International Conference of Harmonization (ICH) guidelines. The method is selective, accurate, precise, and can be used for analysis of pharmaceutical preparations in quality control.

Keywords

Nicergoline, RP-HPLC Method, Validation, Quality Control

Cite This Article

Vania, M. (2014). Development and Validation of a Rapid RP-HPLC Method for Estimation of Nicergoline in Tablet Dosage Forms. International Journal for Pharmaceutical Research Scholars, 3(1), 284-288.

A high performance liquid chromatographic method was developed, validated and applied for determination of Nicergoline in pharmaceutical formulations. A LiChrosorb^® RP-18 (10 µm, 250x 4 mm) column was used with a mobile phase consisting of acetonitrile: methanol: phosphate buffer (40: 35: 25 % v/v, pH 7.0), a quantitative evaluation was performed at 288 nm with flow rate of 1.5 mL/min, and column cooler temperature was maintained at 25 ºC. The retention time was about 8 min. Suitability of this method for the quantitative determination of the drug was proved by validation in accordance with the requirements laid down by the International Conference of Harmonization (ICH) guidelines. The method is selective, accurate, precise, and can be used for analysis of pharmaceutical preparations in quality control.