Abstract

A simple reproducible and efficient   high-performance thin layer chromatographic (HPTLC) method has been developed and validated for the stability study of cabergoline. The method was employed on TLC aluminium plates precoated with silica gel 60 GF₂₅₄ as the stationary phase. The solvent system consisted of chloroform: methanol: ammonia (25%) (80:20:1). This system was found to give compact spot for Cabergoline (R_f value of 0.65-0.03). UV detection was performed at 280 nm. Cabergoline was subjected to acid, alkali, neutral hydrolysis, oxidation, thermal degradation, and photolytic degradation. The drug undergo degradation under acidic and basic conditions, and oxidative degradation. The method was validated for linearity, precision, limit of detection (LOD), limit of quantification (LOQ), specificity and accuracy. Linearity was found to be in the range of 1000- 5000 ng/spot with significantly high value of correlation coefficient r² = 0.990. The LOD and LOQ were 582.2 and 192.14 ng/spot, respectively. As the method could effectively separate the drug from its degradation product, it can employed as a stability indicating one. The recoveries of cabergoline was 99.23%.

Keywords

Bilayer tablet, L-Arginine, SSG, Poly vinyl pyrolidone, HPMC K100M, Light Magnesium Oxide

Cite This Article

Lasan, V.M., Indrekar, T.D. (2016). Development and Validation of Stability Indicating Analytical Method for the Determination of Cabergoline, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(2), 141-150.