Abstract

The aim of present study was to formulate and evaluate Nepafenac liposomal in situ gel. Liposomes were formulated in different ratios using soya lecithin and cholesterol using Rotary Flash Evaporation method. Liposomes were evaluated for drug entrapment efficiency, %entrapment efficiency, particle size analysis, zeta potential and in vitro release studies. Optimized liposomes (F4) were developed into in situ gels. The thermo reversible liposomal in situ gels of Nepafenac were  prepared  by  using  poloxamer  407  and  with mucoadhesive  polymers  like  HPMC  E15 and chitosan. Formulations were sterilized by autoclaving at 121⁰C, at 15 lb pressure for 20 min. The formulations were  evaluated  for  drug  content,  clarity,  pH, gelation temperature,  gelation  capacity,  viscosity,  in-vitro  drug  release studies. Drug and Polymer incompatibilities were evaluated using FTIR spectrophotometer. The drug content of the formulations was in the range 83%-92%. pH of the formulations was in the range 6.43 to 7.42, gelation temperature was in the range 27.5⁰C-40⁰C. In Vitro drug release was in the range of 78%-99% within 12 hours, the extent of gelation and consequently the release of Nepafenac depended on the concentration of polymers used. Nepafenac was released slowly from gels, for a period of 12 hours. Formulation PC5 with poloxamer 407 (18% w/w) and chitosan (0.3% w/w) was found to be suitable as it released 78% of drug for a period of 12 hours. Poloxamer 407/chitosan (PC5) combination was found to have optimum pH and gelation temperature which is required for an in situ gel drug delivery system.

Keywords

In Situ Gel, Liposomes, Entrapment Efficiency, Zeta Potential

Cite This Article

Nalla, A., Panigrahy, R.N., Chinnala, K.M. (2016). Formulation Development and Evaluation of Nepafenac Novel In Situ Gel, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(4), 1-14.