Abstract

The present study was undertaken to evaluate the dose – dependent toxic effect of diethyl phthalate (DEP) in liver of mice and its amelioration by extract of Nigella sativa. Healthy adult Swiss strain female albino mice were orally administered with DEP (310, 620 and 1240 mg/kg body weight/day) and DEP (1240 mg/kg body weight/day) plus Nigella sativa seed extract (150 and 300 mg/kg body weight/day) for 30 days. Various parameters were evaluated. The results revealed significant (p < 0.05) elevation in hepatic lipid peroxidation, which could be due to significant (p < 0.05) reduction in non-enzymatic (glutathione and total ascorbic acid contents) and enzymatic (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione transferase activities) antioxidants. The effect was dose – dependent. Oral administration of Nigella sativa seed extract (150 and 300 mg/kg body weight/day) with DEP (High dose) for 30 days resulted in dose – dependent and significant (p<0.05) reduction in lipid peroxidation as compared to DEP alone treated group. Similarly, all doses of Nigella sativa seeds extract ameliorated DEP- induced changes in non – enzymatic and enzymatic antioxidants. Amelioration of DEP- induced changes were maximum by 300 mg/kg body weight/day extract. Results of present study indicate that DEP – caused hepatotoxicity by inducing oxidative stress, whereas antihepatotoxic effect of Nigella sativa seeds extract was mainly due to its antioxidative potency.

Keywords

Diethyl Phthalate, Nigella Sativa, Oxidative Stress, Hepatotoxicity

Cite This Article

Prajapati, H., Verma, R.J. (2015). Nigella sativa Ameliorates Diethyl Phthalate - Induced Hepatotoxicity, International Journal for Pharmaceutical Research Scholars (IJPRS), 4(1), 112-119.