Abstract

A simple, precise and accurate liquid chromatographic method was described for simultaneous determination of paracetamol, diclofenac sodium and famotidine in tablets. The chromatography was carried out on a C₁₈ (250 mm x 4.6 mm, 10 μm) column with acetonitrile:water: 0.5% trimethylamine : o-phosphoric acid (60:20:5:15v/v) as mobile phase, at a flow rate of 1.0 ml/min, with detection at 240 nm. Separation was completed in less than 10 min. The calibration curves were linear in the concentration range of 125.0-1000.0 μg/ml for paracetamol, 25.00-200.0 μg/ml for diclofenac sodium and 5.00-40.00 μg/ml for famotidine. The intra- and inter-day relative standard deviations for both the components were <2.0 %. The results of the studies showed that the proposed RP-HPLC method is rapid, precise and accurate, which can be applied for the routine assessment of described drugs in pharmaceutical dosage forms.

Keywords

Paracetamol, Diclofenac Sodium, Famotidine, Simultaneous Determination, RP-HPLC, Validation

Cite This Article

Maslarska, V.N., Tsvetkova, B.G., Peikova, L.P. (2015). RP-HPLC Method for Simultaneous Determination of Paracetamol, Diclofenac Sodium and Famotidine in Tablet Dosage Form, International Journal for Pharmaceutical Research Scholars (IJPRS), 4(2), 55-61.