Abstract

A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Phenylephrine HCl, Guaiphenesin, Bromhexine HCl and Cetirizine Hydrochloride, in combined dosage form. A Qualisil, C18 column, 250 mm x 4.6 mm, 5µm in isocratic mode with mobile phase containing 0.05M KH₂PO₄-1.0% HCl Buffer: ACN (62: 38) pH was adjusted to 2.5 by TEA. The flow rate 1.0ml/min and effluents were monitored at 254nm. The retention time of the Phenylephrine HCl, Guaiphenesine, Bromhexine HCl and Cetirizine Hydrochloride was found to 2.78, 3.88, 6.90 and 8.07 mins. respectively. The different analytical parameters such as accuracy, precision, robustness, limit of detection and limit of quantification were determined  according to the International Conference on Harmonization (ICH) Q2R1 guidelines. The detector response was linear in the range of 0.2-1.0 μg/ml, 2-10 μg/ml, 0.16-0.8 μg/ml and 0.1-0.5 μg/ml, for Phenylephrine HCl, Guaiphenesine, Bromhexine HCl and Cetirizine Hydrochloride respectively. The proposed method was successfully applied for the simultaneous estimation of all drugs in pharmaceutical dosage forms.

Keywords

RP-HPLC, Cetirizine Hydrochloride, Bromhexine Hydrochloride, Phenylephrine Hydrochloride, Guaiphenesin, Validation, Cough formulation and method development

Cite This Article

Sheikh, S., Asghar, S., & Ahmad, S. (2013). RP-HPLC Method for the Simultaneous Estimation of Phenylephrine Hydrochloride, Guaiphenesin, Bromhexine Hydrochloride and Cetirizine Hydrochloride in Pharmaceutical Liquid Formulation. International Journal for Pharmaceutical Research Scholars (IJPRS), 2(4), 448-457.