Abstract

Two simple, sensitive, accurate, precise, rapid and economical methods were developed and validated for the quantitative determination of simultaneous estimation of Loteprednol etabonate (LOTE) and Gatifloxacin (GAT) in bulk and ophthalmic formulations. Method I is based on simultaneous equations and Method II on Q-absorbance ratio. The absorption maxima were found to be at 243nm and 291nm for LOTE and GAT and Isoabsorptive point at 267nm. Beer's law is obeyed in the concentration range of 3-18 µg/ml and 2-12 µg/ml for LOTE and GAT respectively. The label claim of LOTE and GAT was found to be 101.30% and 103.05% for method I and 102.50% and 98.66% for method II. The methods was successfully applied to pharmaceutical dosage form because no interference from the synthetic mixture excipients was found. The suitability of this method for the quantitative determination of LOTE and GAT was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of LOTE and GAT in pharmaceutical dosage form. The results of analysis have been validated statistically and by recovery studies.

Keywords

Loteprednol etabonate, Gatifloxacin, Simultaneous Equation Method, Q-absorption Method, Isoabsorptive Point, Validation

Cite This Article

Premakumari, K. B., Murugan, V., & Nargund, L. V. G. (2014). Simultaneous Spectrophotometric Estimation of Loteprednol Etabonate and Gatifloxacin in Bulk and Ophthalmic Formulation. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 482-490.