Abstract

Standardized herbal products of consistent quality and containing well defined constituents are required for reliable clinical trials and to provide consistent beneficial therapeutic effects. Pharmacological properties of an herbal formulation depend on phytochemical constituents present therein. Development of authentic analytical methods which can reliably profile the phytochemical composition, including quantitative analyses of marker/bioactive compounds and other major constituents, is a major challenge to scientists. Agnitundi Vati is an Ayurvedic tablet used in indigestion and fever. In the present work, attempt has been made to develop a chromatographic method for standardization of Agnitundi vati. Strychnine in Strychnos nux vomica and Gallic acid in Terminalia chebula, Terminalia bellirica, Emblica officinalis are selected as marker compounds. A simple, rapid, precise, accurate and reproducible high performance thin layer chromatographic (HPTLC) method was developed for quantification of strychnine and Gallic acid in Agnitundi vati. The proposed method was validated as per ICH guidelines.

Keywords

Agnitundi Vati, Strychnine, Gallic Acid, HPTLC, Polyherbal Formulations, ICH

Cite This Article

Khanvilkar, V.V., Ayare, P.P., Kadam, V.J. (2016). Standardization of Agnitundi Vati: An Ayurvedic Polyherbal Formulation, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(1), 75-83.