Ganipisetty, L. A., Dachinamoorthy, D., Rao, J. V. L. N.
A simple, accurate, sensitive and validated RP-HPLC method for simultaneous determination of Losartan and hydrochlorothiazide in combined tablet dosage form has been developed. Separation carried out on RP-HPLC system equipped with Inertsil ODS -3V Column (150 × 4.6 mm i.d., 5µm particle size) using mobile phase of Acetonitrile and phosphate buffer adjusted pH to 2 with orthophosphoric acid at a flow rate of 1 mL/min in the Gradient program with run time 25 minutes and detection using UV/VIS detector was carried out at 226 nm. Results were linear in the range of 20–150 μg/mL and 5-40 μg/mL for losartan and hydrochlorothiazide respectively. The method has been successfully applied for the analysis of drugs in pharmaceutical formulation. Results of analysis were validated statistically and by recovery studies.
RP-HPLC, Losartan, Hydrochlorothiazide, Tablet Dosage Form
Cite This Article
Ganipisetty, L. A., Dachinamoorthy, D., Rao, J. V. L. N. (2014). A Sensitive RP-HPLC Method Development and Validation for the Simultaneous Estimation of Losartan Potassium and Hydrochlorothiazide. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 408-416.