A Study on Anti-Ulcer Activity of Diospyros Malabarica Bark in Ulcer Induced Rats

Author(s)

Gopalakrishna, C. H., Reddy, A. K. D., Sharief, N., Sushma, K. R., Narendra S.

Author's Affiliation

Abstract

Peptic ulcer is a common gastrointestinal disease and it affects particularly the working years of a patient’s life and its social implications are considerable. In the indigenous system of medicine, the DM bark is reported to be useful in the treatment of gastric ulceration. Hence, in the present study, the DM bark has been selected for its anti-ulcer potency on experimentally induced ulcer in rats. The authenticated DM bark, were dried in shade and powdered coarsely. Extraction was done according to standard procedure using analytical grade solvents. The coarse powder of DM bark was Soxlet extracted with the solvents with increasing order of polarity i.e. petroleum ether (60-80^oC), chloroform (59.5-61.5^oC), ethanol (64.5-65.5^oC), and distilled water. The extracts obtained were concentrated under reduced pressure. In the pharmacological screening, the effect of different extracts of DM bark was evaluated for their anti-ulcer profile by using Pylorus ligation, and ethanolic induced models using albino rats. Preliminary phytochemical investigation revealed the presence of flavonoids, carbohydrates, proteins, steroids, tannins and glycosides in chlorofom extract. LD₅₀ cut-off dose was found to be 2000 mg/kg b.w. for the extracts of DM bark. Hence therapeutic dose was taken as 500 mg/kg b.w. for all extracts. Treatment with chlorofom extract of DM bark significantly showed the anti-ulcer activity as compared to control. The results were comparable to that of standard drug (Lansoprazole). From the literature survey and the work carried out, it may be confirmed that DM bark does possesses anti-ulcer property. Phytochemical investigation suggests the presence of flavonoids, tannins which may be responsible for the anti-ulcer activity.

Keywords

Ulcer, Diospyros Malabarica, Extraction, Flavonoids, Pylorus Ligation, Chlorofom, Lansoprazole

Cite This Article

Gopalakrishna. CH, Ashok Kumar Reddy. D, Sharief N, Sushma KR, Narendra S. (2014). A Study on Anti-Ulcer Activity of Diospyros Malabarica Bark in Ulcer Induced Rats. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 375-381.