A Validated Visible Spectrophotometric Method for the Assay of Moxifloxacin Tablet Dosage Forms Using Fe (III) in Buffer Media

Author(s)

Ramanna, P., Saritha, B., Reddy, T. S.

Author's Affiliation

Abstract

Moxifloxacin reacts with Fe(III) in the pH range 1.0-4.0 to form a yellow colored complex solution.  Its absorption spectrum shows maximum absorbance at 440 nm. The absorbance is constant and maximum in the pH range 2.0-3.0. pH 2.5 is selected for analytical studies. The absorbance of the complex solution varied linearly with the amount of moxifloxacin. A plot of   the amount of moxifloxacin and the absorbance at 440 nm is linear and obeys the equation A₄₄₀ = 0.0317 C + 0.0004. The linear plot shows that Beer’s law is obeyed in the range 2.0-32.0 µg/ml of moxifloxacin. The molar absorptivity is 1.286 x 10⁴ l mol^-1 cm^-1. The Sandell’s sensitivity is 0.0314 µg cm^-1. The standard deviation of the method for ten determinations of 10 µg/ml of moxifloxacin   is 0.0017. The correlation coefficient (g) is 0.9999. The effect of excipients that are generally associated with moxifloxacin in pharmaceutical dosage forms is investigated. The proposed visible spectrophotometric method was validated as per ICH guidelines. The validation parameters such as, linearity, accuracy, precision, LOD, LOQ and ruggedness were investigated. The method is simple, rapid, precise, selective and accurate. The present method was applied for the determination of moxifloxacin in its tablet dosage forms.

Keywords

Moxifloxacin, Fe (III), Visible Spectrophotometry, Method Validation, Buffer Media

Cite This Article

Ramanna, P., Saritha, B., & Reddy, T. S. (2014). A Validated Visible Spectrophotometric Method for the Assay of Moxifloxacin Tablet Dosage Forms Using Fe (III) in Buffer Media. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(3), 202-207.