Patel, R. C., Dixit, P. B., Vyas, H. A., Patel, N. K., Sharda, A. M.
Liquid chromatography–solid phase extraction–nuclear magnetic resonance (HPLC-SPE-NMR), novel and highly promising hyphenated technique which is based on post-column analyte trapping by solid-phase extraction. The analytes are subsequently eluted from the SPE cartridges using deuterated solvents. This indirect HPLC-NMR hyphenation offers numerous advantages compared to direct HPLC-NMR methods. Multiple trapping leads to a dramatic increase of analyte amounts available for NMR, enabling acquisition of high-quality 2D NMR data within a short time. The well-defined NMR solvent conditions make spectra comparisons feasible, which means databases and spectra catalogues.
HPLC, Solid Phase Extraction (SPE), NMR
Cite This Article
Patel, R. C., Dixit, P. B., Vyas, H. A., Patel, N. K., Sharda, A. M. (2013). An Introduction to a Hyphenated Technique: HPLC-SPE-NMR. International Journal for Pharmaceutical Research Scholars (IJPRS), 2(2), 47-50.