Mishra, S., Arora, V.
A novel, accurate and precise HPLC method for determination of assay of Anti-cholinergic drug has been validated. Separation was achieved on Inertsil ODS-3, 3µm using Buffer:ACN:Methanol as mobile phase at a flow rate of 1.2 ml/min and UV detection at 222 nm. The developed method was applied for determination of assay of Anticholinergic drug in Parenteral formulation and the method was validated with respect to Specificity, Precision, Linearity, Accuracy, Robustness and analytical solution stability. The method was linear over the range of 60-140 µg/ml for Glycopyrrolate. The mean recovery was found to be in the range of 99.12-99.73 %. The percentage of relative standard deviation was found to be less than critical value. The method was found to be accurate, precise and selective for simultaneous estimation of Glycopyrrolate in injections.
Glycopyrrolate, Reverse-phase HPLC, Method Validation
Cite This Article
Mishra, S., Arora, V. (2016). Analytical Method Validation of HPLC Method for Assay of Anticholinergic Drug in Parenteral Formulation, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(4), 101-104.