Cramer’s Rule, Tri Linear Regression and RP-HPLC Method Development for the Estimation of Combined Dosage Form of Anti Diabetic Drugs

Author(s)

Bhauvaneswara, R.C., Deepthi, G., Polampalli, L., Yadav, R.K., Manikanta, G.

Author's Affiliation

Abstract

In this study two simple, rapid, precise and accurate spectrophotometric methods and one RP – HPLC method were developed and validated for the estimation of Atorvastatin calcium, Metformin hydrochloride and Glimepiride in pure form and in tablet dosage form. Different aliquots of Atorvastatin calcium, Metformin hydrochloride and Glimepiride in methanol were prepared in the concentration range of 4 – 24, 3 – 15 and 1 - 5 µg/ml respectively. The percentage label claim present in tablet formulation was found to be 98.46 ± 1.2781, 102.29 ± 1.1598 and 99.67 ± 1.1832 % for Atorvastatin calcium, Metformin hydrochloride and Glimepiride, respectively. The accuracy of the method was confirmed by recovery studies. The % RSD value, percentage recovery for Atorvastatin calcium, Metformin hydrochloride and Glimepiride were calculated.

Keywords

Atorvastatin Calcium, Metformin Hydrochloride, Glimepiride, Percentage Recovery Studies, Regression, Chromatography

Cite This Article

Bhauvaneswara, R.C., Deepthi, G., Polampalli, L., Yadav, R.K., & Manikanta, G. (2016). Cramer’s Rule, Tri Linear Regression and RP-HPLC Method Development for the Estimation of Combined Dosage Form of Anti Diabetic Drugs, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(2), 14-45.