Derivative Spectrophotometric Method for Simultaneous Determination of Granisetron and Pantoprazole in Synthetic Mixture

Author(s)

Patel, S.R., Patel, N.K., Patel, D.B., Patel, P.A., Patel, L.J.

Author's Affiliation

Abstract

The present work describes a First order Derivative Spectrophotometric method for simultaneous estimation of Granisetron and Pantoprazole in synthetic mixture. Method was performed on Elico’s Double beam UV-Visible Spectrophotometer (SL-191) using methanol as a solvent. Absorbance were recorded at Zero Crossing Point (ZCP) of Granisetron (248 nm) and ZCP of Pantoprazole (291 nm) for all standard and sample solutions. The selected Spectrophotometric conditions were found to be effective for the determination of Granisetron and Pantoprazole from synthetic mixture in presence of common excipients without prior physical separation. Linearity was found over the range of 2-20 μg/ml for Granisetron and over 5-100 μg/ml for Pantoprazole. The values of Limit of Detection were found to be 0.40 μg/ml for Granisetron and 0.62 μg/ml for Pantoprazole. The values of Limit of Quantification were found to be 1.22 μg/ml for Granisetron and 1.89 μg/ml for Pantoprazole. The proposed method was found to be fast, simple, sensitive, accurate, precise, reproducible, robust and rugged and can be used for simultaneous analysis of these drugs in synthetic mixture.

Keywords

Granisetron, Pantoprazole, Derivative Spectroscopy

Cite This Article

Patel, S.R., Patel, N.K., Patel, D.B., Patel, P.A., Patel, L.J. (2012). Derivative Spectrophotometric Method for Simultaneous Determination of Granisetron and Pantoprazole in Synthetic Mixture, International Journal for Pharmaceutical Research Scholars (IJPRS), 1(2), 115-121.