Development and Validation of Difference Spectrometric Method for the Estimation of Cidofovir Dihydrate in Bulk and Pharmaceutical Formulation

Author(s)

Nakum, R.V., Mardia, R.B., Chauhan, S.P., Suhagia, B.N.

Author's Affiliation

Abstract

A simple, specific and rapid difference spectroscopic method has been developed for the estimation of Cidofovir dihydrate in bulk and Pharmaceutical formulation. The proposed method was carried out by measuring the difference absorbance of Cidofovir dihydrate in two different conditions containing three different forms of drug generated by neutral (solvent), acidic (solvent) and basic (solvent) medium. The measurements of difference absorbance were carried out at 290 nm for two different conditions. The calibration curves were linear in the concentration range of 5- 25µg/ml. The proposed method was validated as per ICH validation guideline Q₂(R₁) for accuracy, robustness, LOD, LOQ etc. The method was found to be accurate, precise, robust and sensitive hence it can be applied in routine analysis of Cidofovir dihydrate in bulk and Pharmaceutical formulation in its quality control.

Keywords

Difference spectrometry, Cidofovir, Validation

Cite This Article

Nakum, R.V., Mardia, R.B., Chauhan, S.P., Suhagia, B.N. (2015). Development and Validation of Difference Spectrometric Method for the Estimation of Cidofovir Dihydrate in Bulk and Pharmaceutical Formulation, International Journal for Pharmaceutical Research Scholars (IJPRS), 4(2), 244-249.