Development and Validation of RP-HPLC Method for Simultaneous Determination of Amlodipine and Lisinopril in Pharmaceutical Dosage Form

Author(s)

Aswini, G. L., Dachinamoorthy, D., Rao, J. V. L. N.

Author's Affiliation

Abstract

A simple, accurate, sensitive and validated RP-HPLC method for simultaneous determination of Amlodipine Besylate and Lisinopril in combined tablet dosage form has been developed. Separation carried out on RP-HPLC system equipped with Zorbax SB-C8 Column (150 × 4.6 mm i.d., 3.5µm particle size) using mobile phase of Acetonitrile and phosphate buffer adjusted pH to 3.6 with orthophosphoric acid at a flow rate of 1 mL/min in the Gradient program with run time of 10 minutes and detection using UV/VIS detector was carried out at 210 nm. Results were linear in the range of 12 – 36 μg/mL for both Amlodipine besylate and lisinopril respectively. The method has been successfully applied for the analysis of drugs in pharmaceutical formulation. Results of analysis were validated statistically and by recovery studies.

Keywords

RP-HPLC, Amlodipine Besylate, Lisinopril, Tablet Dosage Form

Cite This Article

Aswini, G. L., Dachinamoorthy, D., & Rao, J. V. L. N. (2014). Development and Validation of RP-HPLC Method for Simultaneous Determination of Amlodipine and Lisinopril in Pharmaceutical Dosage Form. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(1), 704-712.