Development and Validation of RP-HPLC Method for Simultaneous Estimation of Ramipril and Chlorthalidone in Bulk and Combined Dosage Form

Author(s)

Makwana, C. J., Patel, P. D., Thummar, J. M., Dey, S., Upadhyay, U. M.

Author's Affiliation

Abstract

Ramipril is angiotensin-converting enzyme inhibitor. Chlorthalidone is one of the oldest and widely used thiazide diuretics. Simple, accurate and economic RP-HPLC method have been described for the simultaneous estimation of Ramipril and Chlorthalidone in bulk and combined dosage form. Reverse Phase high-performance liquid chromatographic method was developed for the determination of Ramipril and Chlorthalidone using Acetonitrile: Water (40:60 v/v) of as the mobile phase and measuring the response at λmax 215 nm. The analysis was performed on a C18 (250X4.6 mm ID), 5.0 µ column. The calibration curve was obtained for Ramipril and Chlorthalidone at 2-12 µg/ml and 5-30 µg/ml respectively. The mean recovery was found to be 98.21% and 98.46% for Ramipril and Chlorthalidone respectively. The LOD and LOQ value for ramipril was found to be 0.069 and 0.218 respectively. The LOD and LOQ value for Chlorthalidone was found to be 0.0076 and 0.025 respectively. The method was validated according to the ICH guidelines.

Keywords

Ramipril, Chlorthalidone, RP-HPLC

Cite This Article

Makwana, C. J., Patel, P. D,, Thummar, J. M., Dey, S., & Upadhyay, U. M. (2014). Development and Validation of RP-HPLC Method for Simultaneous Estimation of Ramipril and Chlorthalidone in Bulk and Combined Dosage Form. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 28-37.