Development and Validation of Stability Indicating Method for Minoxidil and Finasteride in its Pharmaceutical Dosage Form

Author(s)

Patel, P.D., Surti, N.I., Upadhyay, U.M.

Author's Affiliation

Abstract

A simple, accurate, precise and specific RPHPLC method has been developed and validated for simultaneous estimation of Finasteride and Minoxidil in its Pharmaceutical dosage form. RPHPLC method was optimized on Hypersil BDS C-18, 250mm x 4.6mm, 5µ particle size with mobile phase Phosphate Buffer (pH 7) : ACN in ratio of (80:20 v/v) and pH 7 adjusted with 1 M NaOH. The mobile phase at flow rate of 1.0 ml/min, Injection volume 20µl and detection wavelength was kept 216 nm. The retention time for Finasteride and Minoxidil was 2.95±0.1 min and 5.74±0.1 min respectively. The linearity was observed in the concentration range of 0.50 to 1.50 mcg/ml and 25 to 75 mcg/ml with correlation coefficient of 0.997 and 0.999 for Finasteride and Minoxidil respectively. The % degradation during force degradation was found to be 10 to 50 % for both Finasteride and Minoxidil in the given condition using developed RP- HPLC method.

Keywords

Minoxidil, Finasteride, RP-HPLC, Stability Indicating, Validation

Cite This Article

Patel, P.D., Surti, N.I., Upadhyay, U.M. (2015). Development and Validation of Stability Indicating Method for Minoxidil and Finasteride in its Pharmaceutical Dosage Form, International Journal for Pharmaceutical Research Scholars (IJPRS), 4(2), 221-238.