Evaluation of Ivabradine against Insulin Resistance Syndrome in Experimental Animals

Author(s)

Karia, P. D., Patel, K. V., Gandhi, T. R.

Author's Affiliation

Abstract

Insulin resistance syndrome comprises of high glucose level, hypertension and dyslipidemia. The present study was aimed to evaluate effect of Ivabradine in insulin resistance syndrome in experimental animals. Male Sprague-Dawley rats of 6-8 weeks (150-180g) were randomly allocated based on serum glucose levels in 8 groups (n=6). All groups except normal control were fed high fructose diet (HFD) along with drugs for 28 days. Body weight, food intake, mean blood pressure and serum lipid levels were measured weekly. Blood glucose was measured on every 3rd day. On 28^th day, OGTT, serum ions, kidney function markers, antioxidant parameters and histopathology were performed. Statistical analysis was done by ANOVA followed by post hoc Dunnett’s test. Feeding HFD to normal rats for 28 days induced insulin resistance (shown by OGTT) and oxidative stress (increased malondialdehyde, decreased catalase, superoxide dismutase and reduced glutathione) leading to hyperglycemia, dyslipidemia (increased triglyceride, total cholesterol, VLDL, LDL), deteriorated kidney function (increased creatinine , albumin and urea) and hypertension(Systolic BP>130mm/Hg and Diastolic  BP>80mm/Hg). Ivabradine therapy prevented HFD induce insulin resistance and oxidative stress thus leading to improved glycemic control, correction of dyslipidemia, better control of blood pressure and reasonably improved kidney function test. The results were supported by histopathology.

Keywords

Fructose, Insulin Resistance, Hypertension, Dyslipidemia, Ivabradine

Cite This Article

Karia, P. D., Patel, K. V., & Gandhi, T. R. (2014). Evaluation of Ivabradine against Insulin Resistance Syndrome in Experimental Animals. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(4), 248-259.