Sahoo, S., Mishra, S. K., Panda, P. K.
A simple, precise and rapid high performance liquid chromatography method is developed for the simultaneous quantitative determination of Telmisartan and Chlorthalidone from their combination drug product. It involves a Xterra 150 mm x 4.6 mm, 5μm, C-18 column. The separation is achieved on a simple isocratic method. The mobile phase contains a mixture of potassium dihydrogen phosphate buffer pH 2.5 (0.025M): acetonitrile in the ratio 60:40, v/v. The flow rate is 1.0 mL min−1 and the column is maintained at normal temperature. The detector wavelength is 235 nm. The retention times of Chlorthalidone and Telmisartan are 2.5 minutes and 4.4 minutes respectively. The total runtime for the separation of the two active compounds is 6.0 minutes. The described method is validated with respect to system suitability, specificity, linearity, precision and accuracy.
HPLC, Telmisartan, Chlorthalidone, HPLC, Validation
Cite This Article
Sahoo, S., Mishra, S. K., & Panda, P.K. (2012). HPLC Method Development for Simultaneous Estimation of Telmisartan and Chlorthalidone in Tablet Dosage Form, International Journal for Pharmaceutical Research Scholars (IJPRS), 1(4), 1-5.