In-Vitro and In-Vivo Anti-Hepatotoxic Evaluation of Curcuma Aromatica on D-Galactosamine Induced Toxicity

Author(s)

Ammayappan, S. R., Navangul, M. V., Vaithiyalingam, V. J.

Author's Affiliation

Abstract

Curcuma aromatica belongs to the family zingiberaceae. The dried rhizome of Curcuma aromatica was extracted with different solvents like petroleum ether, Toluene, Chloroform, Ethyl acetate, Acetone, Ethanol, Water,. The Phytochemical studies of extracts showed the presence of terpenoids, flavonoids, tannins, alkaloids, saponins and protein and amino acids. Toluene extract of Curcuma aromatica has shown high Total Phenol content, 265±1.08 mg/g which is expressed in terms of Gallic acid and high total flavonol content, 175±1.56 mg/g expressed in terms of rutin. Toluene extract of Curcuma aromatica has shown potent antioxidant activity with IC₅₀ value of 50.62±0.998 µg/ml, with IC₅₀ value of 75±0.87 with IC₅₀ value of 43.75±1.24 µg/ml with IC₅₀ value of 0.038±1.54µg/ml in DPPH, LPO method, in the Scavenging of Hydrogen Peroxide Radicals method and in the ABTS Radical Scavenging Method respectively. Toluene extract at concentration of 200 to 800 µg/ml showed a significant restoration of the altered biochemical parameters towards the normal and it was comparable with standard silymarin, using D- Galactosamine as toxicant. Toluene extract was found to have dose dependent increase in percentage viability of the cells. The 200 and 400 mg/kg b.w toluene extracts of Curcuma aromatica showed a significant restoration of enzyme levels in in-vivo studies. The results were encouraging to state that the hepatoprotective activity exhibited by the toluene extracts of Curcuma aromatica was found to be nearly equivalent with standard silymarin.

Keywords

Curcuma Aromatica, Antihepatotoxic, D-Galactosamine, Antioxidant, Silymarin

Cite This Article

Ammayappan, S. R., Navangul, M. V., & Vaithiyalingam, V. J. (2014). In-Vitro and In-Vivo Anti-Hepatotoxic Evaluation of Curcuma Aromatica on D-Galactosamine Induced Toxicity. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(4), 153-164.