Mosquito Larvicidal Activity of Leaf Extracts of Plant Amomum Subulatum Roxb. Against Anopheles Stephensi (Diptera: Culicidae)

Author(s)

Sharma, B., Vasudeva, N.

Author's Affiliation

Abstract

Dengue, malaria, yellow fever, schistosomiasis, filariasis and Japanese encephalitis are the major health diseases which are transmitted by mosquitoes. To prevent the health problem caused by mosquito vectors and to enhance quality of public health and environment, mosquito control is essential. Plants have immense sources of bioactive components that can be develop to form environmentally friendly and safe vectors control agents. The larvicides play a vital role in preventing mosquitoes in their breeding sites, these also have negative effect in areas of beneficial and non-target organisms.  The leaves of the plant Amomum subulatum were collected and extracted with different solvents such as chloroform, methanol, ethyl acetate and hexane. The larvicidal activity of leaf extracts of the plant was evaluated as per the method recommended by WHO. Batches of 25 third instar larvae were transferred to a small disposable test cups, each containing 200 ml of water. Six replicate were set to for each concentration and an equal number of control were set up simultaneously using tap water. To this 1 ml of appropriate solvent was added. Crude extracts concentration ranging from 60 to 300 ppm was tested. The average larval mortality data were subjected to probit analysis for calculating LC50, LC90. The present investigation has important implications in controlling the mosquito larvae in the polluted aquatic ecosystem. The leaf extracts of Amomum subulatum have remarkable larvicidal activity against the mosquito Anopheles stephensi.

Keywords

Amomum Subulatum, Anopheles Stephensi, Larvicidal Activity, Leaf Extracts

Cite This Article

Sharma, B., Vasudeva, N. (2016). Mosquito Larvicidal Activity of Leaf Extracts of Plant Amomum Subulatum Roxb.  Against Anopheles Stephensi (Diptera: Culicidae), International Journal for Pharmaceutical Research Scholars (IJPRS), 5(3), 26-31.