Spectral Identification and In –Vitro Antioxidant Evaluation of All Trans-Isomers of Lycopene from Solanum lycopersicum Seedless Paste

Author(s)

Wani, R.D.

Author's Affiliation

Abstract

Current study performed on seedless paste of Solanum lycospercium for achieving isolation of Trans-lycopene and searching its antioxidant effects by in-vitro method. Trans-lycopene compound went through Spectral evaluation like UV, IR, GC-MC and PMR where molecular validity of Lycopene checked on functional group and on its structural traits. TLC performed in Dichloromethane-Pet Ether. Column Chromatography conducted to isolate high yield of Trans-lycopene compounds. It was 5.2mg/10g of Trans-Lycopene from Seedless Paste. In-Vitro study carried on this Trans-Lycopene using DPPH Radical, Nitric Oxide assay. It shows good dose dependent antioxidant effect. IC₅₀ value was found to be 20.60 µg/ml & 18.21µg/ml for DPPH & Nitric Acid Assay. It indicates that seedless paste gives not only good yield of pure Trans- lycopene but also proves equivalent antioxidant potency to that of Standard Ascorbic Acid.

Keywords

Trans-lycopene, UV, IR, GC-MS, Nitric Oxide Scavenging

Cite This Article

Wani, R.D. (2017). Spectral Identification and In –Vitro Antioxidant Evaluation of All Trans-Isomers of Lycopene from Solanum lycopersicum Seedless Paste. , 6(1), 161-169.