Spectrophotometric Method Development and Validation for Estimation of Gatifloxacin and Prednisolone Acetate in Bulk and Eye Drop

Author(s)

Jugalkishor, V.B., Pooja, R.B., Sourabh, S.B., Akshay, S.A., Tilottama, T.B.

Author's Affiliation

Abstract

UV spectrophotometric method has been developed and validated for the determination of Gatifloxacin and Prednisolone Acetate in bulk and eye drop. The λmax of Gatifloxacin and Prednisolone Acetate were found to be 292 nm and 242nm, respectively, in methanol. Beer`s law was obeyed in the conc. range of 0.6-3µg/ml and 2-10µg/ml with correlation coefficient of 0.999 and 0.999 for Gatifloxacin and Prednisolone Acetate, respectively. The result of analysis has been validated as per the ICH guidelines. The developed method is simple, selective and reproducible and can be used for routine analysis of formulations containing Gatifloxacin and Prednisolone Acetate.

Keywords

UV Spectrophotometric method, Gatifloxacin, Prednisolone acetate

Cite This Article

Jugalkishor, V.B., Pooja, R.B., Sourabh, S.B., Akshay, S.A., Tilottama, T.B. (2018). Spectrophotometric Method Development and Validation for Estimation of Gatifloxacin and Prednisolone Acetate in Bulk and Eye Drop. International Journal for Pharmaceutical Research Scholars (IJPRS), 7(1), 107-112.