Two simple spectrophotometric methods have been developed for simultaneous estimation of Thiocolchicoside and Dexketoprofen trometamol from a pharmaceutical dosage form. Method-I involved simultaneous equation method and Method-II is the Q-absorbance method. For the simultaneous equation method, the absorbances of the standard solutions were taken at two wavelengths 368 nm (λ-max of Thiocolchicoside) and 258 nm (λ-max of dexketoprofen trometamol). For Q-absorbance method, the absorbances of the standard solutions were taken at two wavelengths 258 nm (λ-max of dexketoprofen trometamol) and 281 nm (isoabsorptive point), in methanol. Linearity range was found to be 2-24 μg/ml for dexketoprofen trometamol and Thiocolchicoside in both methods based on the ratio of the two drugs in combined dosage form. The accuracy and precision of the methods were determined and validated statistically. Both methods showed good reproducibility and recovery with RSD less than 2. Proposed methods were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of dexketoprofen trometamol and Thiocolchicoside in the pharmaceutical dosage form.
Trivedi, J.B., Chaudhari, B.G. (2012). Spectrophotometric Methods for Simultaneous Estimation of Thiocolchicoside and Dexketoprofen Trometamol in Pharmaceutical Dosage Form, International Journal for Pharmaceutical Research Scholars, 1(1), 55-61.
Dexketoprofen trometamol (DKP) chemically, 2-amino-2-(hydroxymethyl) propane-1,3-diol; 2-(3-benzoylphenyl propanoic acid is a water-soluble salt of the dextrorotatory enantiomer or (S)-(+)-enantiomer of the nonsteroidal anti-inflammatory drug (NSAID) ketoprofen1,2. The enantiomer is a relatively new oral NSAID with analgesic, anti-inflammatory and anti-pyretic properties and is one of the most potent in vitro inhibitors of prostaglandin synthesis.