A simple and fast reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the simultaneous determination of ketoprofen – an active component and methylparaben – a preservative, presented in the 2.5 % Ketoprofen gel. The chromatographic separation was performed on a LiChrosorb C18 column; the mobile phase for separation of these compounds consisted of a mixture of 140 ml acetonitrile: methanol (60:40 v/v) and 160 ml 0.5% ammonium acetate. The pH was adjusted to 5.9 with 10 % nitric acid. The analysis time was less than 5 min, at a flow rate 2.0 ml/min and detection at 254 nm. The method was found to be applicable for routine analysis (stability tests, homogeneity) in the pharmaceutical product – Ketoprofen gel 2.5%.
Ketoprofen, Methylparaben, Preservatives, HPLC, Validation
Cite This Article
Maslarska, V. (2014). Stability Indicating HPLC Method for Simultaneous Determination of Ketoprofen and Methylparaben in Gels. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 571-576.