Telomerase and Caspase 3 Expression of Colon Ephitelial Cells Hyperplasia After Andrographis paniculata Nees Extract Administration

Author(s)

Ika, P. N., Imam, S., Junaidi, K.

Author's Affiliation

Abstract

Incidence and death caused by cancer remains high. The anticancer property of andrographolide has been supported by its ability to induce cell cycle arrest at G0-G1. The aim of this study was to prove telomerase and caspase 3 expression of colon cancer cells after Andrographis paniculata Ness extract administration. The female Sprague Dawley rats were treated with oral 7,12-dimethylbenz(a)anthracene (DMBA) 20 mg/kg body weight to induce colon cancer. Twenty five rats were divided into five groups, which were negative control (normal) group, DMBA group, and three groups differed by A.paniculata’s doses (equivalent to 10 mg andrographolide/kg BW, 30 mg/kg BW, and 100 mg/kg BW). Colon tissues was removed after six weeks treatment. From histologic staining with haematoxillyn eosin, it showed that compared to normal group, DMBA induced cell proliferation, nuclear irregularities, and hyperchromatic cells. While the results of immunohystochemistry showed that Andrographis paniculata decreased telomerase and increased caspase 3 expresseion of colon epithelial cells hyperplasia. Andrographis paniculata extract suppressed cancer cell growth by decreased cell proliferation and increased apoptosis.

Keywords

Telomerase, Caspase 3, Colon, DMBA, Andrographis paniculata

Cite This Article

Ika, P. N., Imam, S., & Junaidi, K. (2013). Telomerase and Caspase 3 Expression of Colon Ephitelial Cells Hyperplasia After Andrographis paniculata Nees Extract Administration. International Journal for Pharmaceutical Research Scholars (IJPRS), 2(3), 197-201.