Begum, A., Ahmed, O.
A basic and specific RP-HPLC technique is depicted for the determination of Albuterol and Ipratropium Bromide in dosage forms. Chromatographic separation was accomplished on a C18 column using mobile phase consisting of a mixture of mixed Phosphate buffer pH: 3.4 Methanol (30:70 v/v), with detection of 239 nm and flow rate at 1mL/min. Linearity was observed in the range 36-84 µg /ml for Albuterol (r2 =0.996) & 6-14µg /ml for Ipratropium Bromide (r2 =0.997) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. The proposed strategies were validated. The exactness of the method was evaluated by recovery studies at three distinct levels. Recovery experiments showed the nonattendance of interference from regularly experienced pharmaceutical added substances or additives. The technique was observed to be exact as demonstrated by the repeatability analysis, indicating %RSD less than 2. All statistical data demonstrates legitimacy of the techniques, sensitivity, accuracy or precision and reproducibility. It can be utilized for routine investigation of pharmaceutical dose form.
Assay Studies, Albuterol and Ipratropium Bromide by RP-HPLC
Cite This Article
Begum, A., Ahmed, O. (2016). An Assay Method for the Simultaneous Estimation of Albuterol and Ipratropium Bromide Using RP-HPLC Technology, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(4), 33-37.