Extraction, Isolation and Purification of Solanesol obtained from Nicotiana tobaccum

Author(s)

Thombare, N.A., Dhamak, K.V., Vikhe, B.D., Kshirsagar, S.J.

Author's Affiliation

Abstract

Nicotiana Tobaccum, a polyprenol attracts the attention of bio-chemists because of their significant value as source of isoprene units for the synthesis of metabolically active quinones and Vitamin - K analogues. The present investigative study aims at the isolation of solanesol - a polyprenol from tobacco. The principle of isolation includes the extraction at 50ºC with n-hexane resulting 15-20 percent yield of solanesol. Elution of solanesol with hexane and ethyl acetate (95:5 v/v) has also been identified. The solanesol was further separated from elution by various Chromatographic techniques such as Column Chromatography, TLC, HPLC, produced good yield of solanesol with appreciable percentage of purity.  Modified heat reflux extraction method for extraction and separation of solanesol from tobacco was established and showed better yield than the traditional methods.

Keywords

Solanesol, Extraction, Separation, Silica Gel Column Chromatography, TLC, HPLC

Cite This Article

Thombare, N.A., Dhamak, K.V., Vikhe, B.D., Kshirsagar, S.J. (2015). Extraction, Isolati,on and Purification of Solanesol obtained from Nicotiana tobaccum, International Journal for Pharmaceutical Research Scholars (IJPRS), 4(2), 136-141.