Analytical Method Development and Validation for Simultaneous Estimation of Clotrimazole and Tinidazole by RP-HPLC

Author(s)

Joshi, S., Majmudar, F.

Author's Affiliation

Abstract

An isocratic reverse phase High Performance Liquid Chromatographic (RP-HPLC) method was developed and validated for simultaneous determination of Clotrimazole and Tinidazole in combined pharmaceutical dosage form. The successful separation of Clotrimazole and Tinidazole was achieved using Purospher® C18 (250 mm x 4.6 mm i.d., 5 μm) column, with mobile phase consisting Phosphate buffer (pH 3.5): Acetonitrile (45:55). The mobile phase flow rate was 1.0 ml/min and the detection wavelength was 240 nm. The developed RP-HPLC method was validated according to ICH guidelines with respect to linearity, accuracy, precision, specificity and robustness and also the LOD and LOQ values were determined.

Keywords

Clotrimazole, Tinidazole, RP-HPLC, Validation

Cite This Article

Joshi, S., & Majmudar, F. (2015). Analytical Method Development and Validation for Simultaneous Estimation of Clotrimazole and Tinidazole by RP-HPLC, International Journal for Pharmaceutical Research Scholars (IJPRS), 4(4), 135-140.