In Vitro Efficacy of Piper betle Leaf Extract against Rhizoctonia solani Causing Damping off Disease of Chilli

Author(s)

Beatrice, F.F.S., Santhi, G.

Author's Affiliation

Abstract

In this study, antifungal activity against the pathogen Rhizoctonia solani, Preliminary phytochemical studies and GC-MS analysis of the leaf extracts of Piper betle were carried out. The pathogen was isolated by tissue segment method and identified. Phytochemical analysis of the plant extracts was undertaken. The ethanolic leaf extracts were subjected to GC-MS studies to identify the bioactive compounds. In vitro efficacy of Piper betle leaf extract against pathogen. Antifungal activity of crude extract of P. betle leaves was evaluated against the fungal pathogen R. solani. Hence the bioactive compounds were separated by Gas Chromatography Mass Spectrometry (GC-MS) analysis which revealed the presence of 25 compounds.  All concentration of the leaf extract were found to be inhibitory to growth and the rate of inhibition increased generally by increasing the concentration, 100% concentration was the most effective concentration. This inhibition may be of different bioactive compounds such as Squalene, Dibutyl phthalate, Heptaxyloxane, 9-Octadecenamide, Cyclononasiloxane etc., from P. betle having antifungal properties. This result support an interesting direction of research, the use of plant extracts in controlling disease and is eco-friendly.

Keywords

Rhizoctonia solani, GC-MS, Crude extract, Fungitoxicity, Squalene, Dibutyl phthalate

Cite This Article

Beatrice, F.F.S., Santhi, G. (2017). In Vitro Efficacy of Piper betle Leaf Extract against Rhizoctonia solani Causing Damping off Disease of Chilli. International Journal for Pharmaceutical Research Scholars (IJPRS), 6(1), 109-115.